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Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from zoo and wild animals.
Vet Microbiol. 2018 May; 218:98-103.VM

Abstract

Antimicrobial resistance of Staphylococcus aureus is a major problem in human and veterinary medicine. The aim of this study was to characterise S. aureus isolates from wild and zoo animals mainly associated with bacterial infections. In total, 23 S. aureus isolates, including nine from wild animals and 14 from zoo animals, were obtained during routine diagnostics. All isolates were subjected to multilocus sequence typing (MLST), spa typing, macrorestriction analysis with subsequent SmaI pulsed-field gelelectrophoresis (PFGE), antimicrobial susceptibility testing and S. aureus-specific DNA-microarray analysis. Resistant isolates were also tested for their respective resistance genes by PCR. Isolates from zoo animals and wildlife showed a high diversity of MLST types, spa types and PFGE patterns. Nineteen different spa types were identified, including three novel types and 16 main macrorestriction patterns. Only few isolates were resistant to members of four classes of antimicrobial agents and harboured the respective resistance genes (β-lactams [blaZ, mecA, mecC], tetracyclines [tet(K), tet(L)] and chloramphenicol [catpC221]) or mutations (fluoroquinolones). The DNA microarray analysis identified one isolate from a zoo animal harbouring the toxic shock syndrome toxin gene tst1. Moreover, several enterotoxin genes were detected in five S. aureus isolates. All isolates were negative for Panton-Valentine leukocidin (PVL) genes, but the animal-associated leukocidin genes lukM/lukF-P83 were found in three isolates from two animals.

Authors+Show Affiliations

Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany; Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany. Electronic address: andrea.fessler@fu-berlin.de.Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany; University of Applied Science and Arts, Faculty 2, Department of Bioprocess Engineering, Hannover, Germany.Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany; Federal Institute for Risk Assessment, Berlin, Germany.Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany; Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.Abbott (Alere Technologies GmbH), Jena, Germany; Institute for Medical Microbiology and Hygiene "Carl Gustav Carus", Technische Universität Dresden, Dresden, Germany; InfectoGnostics Research Campus, Jena, Germany.Abbott (Alere Technologies GmbH), Jena, Germany; InfectoGnostics Research Campus, Jena, Germany.Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany; Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29685228

Citation

Feβler, Andrea T., et al. "Phenotypic and Genotypic Characteristics of Staphylococcus Aureus Isolates From Zoo and Wild Animals." Veterinary Microbiology, vol. 218, 2018, pp. 98-103.
Feβler AT, Thomas P, Mühldorfer K, et al. Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from zoo and wild animals. Vet Microbiol. 2018;218:98-103.
Feβler, A. T., Thomas, P., Mühldorfer, K., Grobbel, M., Brombach, J., Eichhorn, I., Monecke, S., Ehricht, R., & Schwarz, S. (2018). Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from zoo and wild animals. Veterinary Microbiology, 218, 98-103. https://doi.org/10.1016/j.vetmic.2018.03.020
Feβler AT, et al. Phenotypic and Genotypic Characteristics of Staphylococcus Aureus Isolates From Zoo and Wild Animals. Vet Microbiol. 2018;218:98-103. PubMed PMID: 29685228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from zoo and wild animals. AU - Feβler,Andrea T, AU - Thomas,Patricia, AU - Mühldorfer,Kristin, AU - Grobbel,Mirjam, AU - Brombach,Julian, AU - Eichhorn,Inga, AU - Monecke,Stefan, AU - Ehricht,Ralf, AU - Schwarz,Stefan, Y1 - 2018/03/20/ PY - 2018/01/24/received PY - 2018/03/13/revised PY - 2018/03/17/accepted PY - 2018/4/25/entrez PY - 2018/4/25/pubmed PY - 2018/11/21/medline KW - Antimicrobial resistance KW - MLST KW - Macrorestriction analysis KW - Microarray analysis KW - spa typing SP - 98 EP - 103 JF - Veterinary microbiology JO - Vet Microbiol VL - 218 N2 - Antimicrobial resistance of Staphylococcus aureus is a major problem in human and veterinary medicine. The aim of this study was to characterise S. aureus isolates from wild and zoo animals mainly associated with bacterial infections. In total, 23 S. aureus isolates, including nine from wild animals and 14 from zoo animals, were obtained during routine diagnostics. All isolates were subjected to multilocus sequence typing (MLST), spa typing, macrorestriction analysis with subsequent SmaI pulsed-field gelelectrophoresis (PFGE), antimicrobial susceptibility testing and S. aureus-specific DNA-microarray analysis. Resistant isolates were also tested for their respective resistance genes by PCR. Isolates from zoo animals and wildlife showed a high diversity of MLST types, spa types and PFGE patterns. Nineteen different spa types were identified, including three novel types and 16 main macrorestriction patterns. Only few isolates were resistant to members of four classes of antimicrobial agents and harboured the respective resistance genes (β-lactams [blaZ, mecA, mecC], tetracyclines [tet(K), tet(L)] and chloramphenicol [catpC221]) or mutations (fluoroquinolones). The DNA microarray analysis identified one isolate from a zoo animal harbouring the toxic shock syndrome toxin gene tst1. Moreover, several enterotoxin genes were detected in five S. aureus isolates. All isolates were negative for Panton-Valentine leukocidin (PVL) genes, but the animal-associated leukocidin genes lukM/lukF-P83 were found in three isolates from two animals. SN - 1873-2542 UR - https://neuro.unboundmedicine.com/medline/citation/29685228/Phenotypic_and_genotypic_characteristics_of_Staphylococcus_aureus_isolates_from_zoo_and_wild_animals_ DB - PRIME DP - Unbound Medicine ER -