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Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans.
Am J Clin Nutr. 2009 Oct; 90(4):1011-22.AJ

Abstract

BACKGROUND

Compared with slow proteins, fast proteins are more completely extracted in the splanchnic bed but contribute less to peripheral protein accretion; however, the independent influence of absorption kinetics and the amino acid (AA) pattern of dietary protein on AA anabolism in individual tissues remains unknown.

OBJECTIVE

We aimed to compare the postprandial regional utilization of proteins with similar AA profiles but different absorption kinetics by coupling clinical experiments with compartmental modeling.

DESIGN

Experimental data pertaining to the intestine, blood, and urine for dietary nitrogen kinetics after a 15N-labeled intact (IC) or hydrolyzed (HC) casein meal were obtained in parallel groups of healthy adults (n = 21) and were analyzed by using a 13-compartment model to predict the cascade of dietary nitrogen absorption and regional metabolism.

RESULTS

IC and HC elicited a similar whole-body postprandial retention of dietary nitrogen, but HC was associated with a faster rate of absorption than was IC, resulting in earlier and stronger hyperaminoacidemia and hyperinsulinemia. An enhancement of both catabolic (26%) and anabolic (37%) utilization of dietary nitrogen occurred in the splanchnic bed at the expense of its further peripheral availability, which reached 18% and 11% of ingested nitrogen 8 h after the IC and HC meals, respectively.

CONCLUSIONS

The form of delivery of dietary AAs constituted an independent factor of modulation of their postprandial regional metabolism, with a fast supply favoring the splanchnic dietary nitrogen uptake over its peripheral anabolic use. These results question a possible effect of ingestion of protein hydrolysates on tissue nitrogen metabolism and accretion. This trial was registered at clinicaltrials.gov as NCT00873951.

Authors+Show Affiliations

INRA, CRNH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19692493

Citation

Deglaire, Amélie, et al. "Hydrolyzed Dietary Casein as Compared With the Intact Protein Reduces Postprandial Peripheral, but Not Whole-body, Uptake of Nitrogen in Humans." The American Journal of Clinical Nutrition, vol. 90, no. 4, 2009, pp. 1011-22.
Deglaire A, Fromentin C, Fouillet H, et al. Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans. Am J Clin Nutr. 2009;90(4):1011-22.
Deglaire, A., Fromentin, C., Fouillet, H., Airinei, G., Gaudichon, C., Boutry, C., Benamouzig, R., Moughan, P. J., Tomé, D., & Bos, C. (2009). Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans. The American Journal of Clinical Nutrition, 90(4), 1011-22. https://doi.org/10.3945/ajcn.2009.27548
Deglaire A, et al. Hydrolyzed Dietary Casein as Compared With the Intact Protein Reduces Postprandial Peripheral, but Not Whole-body, Uptake of Nitrogen in Humans. Am J Clin Nutr. 2009;90(4):1011-22. PubMed PMID: 19692493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans. AU - Deglaire,Amélie, AU - Fromentin,Claire, AU - Fouillet,Hélène, AU - Airinei,Gheorghe, AU - Gaudichon,Claire, AU - Boutry,Claire, AU - Benamouzig,Robert, AU - Moughan,Paul J, AU - Tomé,Daniel, AU - Bos,Cécile, Y1 - 2009/08/19/ PY - 2009/8/21/entrez PY - 2009/8/21/pubmed PY - 2009/10/10/medline SP - 1011 EP - 22 JF - The American journal of clinical nutrition JO - Am J Clin Nutr VL - 90 IS - 4 N2 - BACKGROUND: Compared with slow proteins, fast proteins are more completely extracted in the splanchnic bed but contribute less to peripheral protein accretion; however, the independent influence of absorption kinetics and the amino acid (AA) pattern of dietary protein on AA anabolism in individual tissues remains unknown. OBJECTIVE: We aimed to compare the postprandial regional utilization of proteins with similar AA profiles but different absorption kinetics by coupling clinical experiments with compartmental modeling. DESIGN: Experimental data pertaining to the intestine, blood, and urine for dietary nitrogen kinetics after a 15N-labeled intact (IC) or hydrolyzed (HC) casein meal were obtained in parallel groups of healthy adults (n = 21) and were analyzed by using a 13-compartment model to predict the cascade of dietary nitrogen absorption and regional metabolism. RESULTS: IC and HC elicited a similar whole-body postprandial retention of dietary nitrogen, but HC was associated with a faster rate of absorption than was IC, resulting in earlier and stronger hyperaminoacidemia and hyperinsulinemia. An enhancement of both catabolic (26%) and anabolic (37%) utilization of dietary nitrogen occurred in the splanchnic bed at the expense of its further peripheral availability, which reached 18% and 11% of ingested nitrogen 8 h after the IC and HC meals, respectively. CONCLUSIONS: The form of delivery of dietary AAs constituted an independent factor of modulation of their postprandial regional metabolism, with a fast supply favoring the splanchnic dietary nitrogen uptake over its peripheral anabolic use. These results question a possible effect of ingestion of protein hydrolysates on tissue nitrogen metabolism and accretion. This trial was registered at clinicaltrials.gov as NCT00873951. SN - 1938-3207 UR - https://neuro.unboundmedicine.com/medline/citation/19692493/Hydrolyzed_dietary_casein_as_compared_with_the_intact_protein_reduces_postprandial_peripheral_but_not_whole_body_uptake_of_nitrogen_in_humans_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.2009.27548 DB - PRIME DP - Unbound Medicine ER -